Paul andersen explains the two major portions of the molecular biology lab in ap biology. Information regarding ap and ib coursework alma college. Microsoft word ap bio lab06 molecular biology worksheetwl. Objectives before doing this lab you should understand. Big genetics and information transfer 3 ap central. We need to build a lab spreadsheet that will model how allele frequencies change through one life cycle of this imaginary population. The eight themes for the ap curricular requirements are integrated into the instruction, labs, readings, and reports that students must complete. Explain how the principles of gel electrophoresis allow for the separation of dna.
In this exercise you will measure diffusion of small molecules through dialysis tubing, an example of a semi permeable membrane. The photosynthetic rate in plants will be higher in the plants with unboiled chloroplasts in the light. In a population of individuals, 510 show the dominant phenotype. In activity b testing physical fitness, students count pulse rates under different conditions to determine their level of. Ap biology lab manual for teachers supplement technology integration use of gas pressure probeware provides a different means of running lab 2 a more rapidly, b without the necessity of using caustic sulfuric acid or messy permanganate stain and c without timing problems involved in stopping the reaction at specific points. Diffusion and osmosis overview in this lab you will. Trace the edge pattern of the leaves directly on to the grid. Meets ap science practices 1, 3, 5, 6, and 7, and big idea 3. These do not replace reading the textbook, so dont become overly dependent on them. In a diploid organism that has gene a gene loci that each contain one of two alleles for. Cellular respiration essay 1990 the results below are measurements of cumulative oxygen consumption by germinating and dry seeds. In your lab notebook, record data, answers to questions, and any questions that arise.
It was discovered in 1858, by rudolf virchow, that new cells can only arise from previously existing cells. Note that the baseline determined should be approximately the same for all groups. Advanced placement biology ap biology or ap bio is an advanced placement biology course and exam offered by the college board in the united states. They are in a condensed format which has six slides to a page. Ap biology investigative labs ap central the college board. You will use approximately 50 ml of agarose per gel depending upon your electrophoresis apparatus. Some of the recommended labs may be too expensive or too time consuming for your class. One of the characteristics of living things is the ability to replicate and pass on genetic information to the next generation. Remove most of the air from the bag but leave a little bit of space and tie the. Somatic body cells divide exclusively by mitosis followed by cytokinesis, while germ cells produce gametes by the process of. Austin bennett will be attending stanford university in the fall to study computer science. Transformation prelab guided investigation independent inquiry c l i c k o n t h e t o p i c t o g o t o t h a t s e c t i o n pacingteachers notes slide 3 31.
This lab will allow for the exploration of the hardyweinberg law of genetic equilibrium in depth by studying the relationship between evolution and changes in allele frequencies in a sample population, the class. Investigating factors that affect the rate of cellular respiration 1556k. Step 1 form lab teams, as instructed by your teacher. Diffusion and osmosis do not write on this document in this lab, you will observe the. Ap biology syllabus, class procedures course description. Hes an unhooked admin who got into stanford, cmu, duke, etc. Andersen explains hardyweinberg equilibrium and describes. Weinberg developed a theory that evolution could be described as a change of the frequency of alleles in an entire population. Dialysis bag results group data contents in dialysis bag initial mass final mass mass difference percent. The arrows indicate reaction sites for two restriction enzymes enzyme x and enzyme y. Prepare the agarose gel just before the lab and maintain it in its liquid state by placing it in a 5565c water bath. Ap biology lab manual for teachers biology junction. A seed contains an embryo plant and a food supply surrounded by a seed coat.
When the necessary conditions are met, germination occurs, and the rate of cellular respiration greatly increases. Bigidea investigation 1 s17 evolution 1 investigation 1 artificial selection. The objective of this lab is to compare the photosynthetic rates at varying light. He explains how you can use the pglo plasmid to produce glowing e. Obtain data from the other lab groups in your class to complete table 1. Each kit contains sufficient materials to outfit 8 student workstations. Gently arrange your plants leaves on the grid of your lab write up p3. For exercises 8b, 8c, and 8d and optional exercise 8e, draw table 8. Dnas, or hybrids, created by joining pieces of dna from two different sources. Ap biology fi course planning and pacing guide 4 2012 the college board about the college board the college board is a missiondriven notforprofit organization that connects students to college success and opportunity. Then, gently tap the tube to mix the solution and dispose of bio hazard in the waste bag. Assign one student group to complete 2c while other groups are performing 2b.
The technique is much easier for the students to use and gets better results. In this experiment you will work with seeds that are living but dormant. Return your isopods to the stock culture or separate holding area. Cell respiration adapted from ap biology lab manual overview in this experiment, you will work with seeds that are living but dormant. Restriction enzyme cleavage of dna and gel electrophoresis. The objective of this lab is to compare the photosynthetic rates at varying light intensities and wavelengths using a controlled experiment. This is an alternative to the ap enzyme catalysis lab. How many individuals would you expect of each of the possible three genotypes for.
Dec 26, 2017 meets ap science practices 1, 3, 5, 6, and 7, and big idea 3. Describe the strategies organisms use to acquire and use energy. He then describes how you can use restriction enzymes and the process of gel electrophoresis to cut and separate dna. Ap biology hardyweinberg practice problems answer key. Ap bio reading guides biology in focus 2nd edition. Molecular biology adapted from bio rad forensic dna fingerprinting kit lab, vernier ap biology lab manual and college board ap biology lab manual. These laboratory materials have been prepared by carolina biological supply company. Gas volume measurements were corrected for changes in temperature and pressure. Lab report rubric print, attach and selfgrade for every formal lab report that you hand in lab report procedure and grading lab notebook grading rubric print and put in your lab notebook when handing it in for grading. Authors and contributors of the 1990 ap biology lab manual and subsequent editions. Using the information above, ask students to construct a diagram of the arabinose. The detailed lab protocol can be found in the student manual. The main enzyme for this lab, peroxidase, is found in many different forms, with optimum phs ranging from 4 to 11 depending on the source and optimum temperatures varying from 10 to 70c. The ap college board lists labs for its recommended curriculum, however, teachers are not limited to only using their versions of the lab.
Ap biology hardyweinberg practice problems answer key 1. Molecular biology essay 1995 the diagram below shows a segment of dna with a total length of 4,900 base pairs. Cover the top of the agarose container flask to minimize evaporation. Students complete 2a and perform the first part of 2c. Each gram of a plants dry biomass is equivalent to 4.
The ap designation is a registered trademark of the college board. You have sampled a population in which you know that the percentage of the homozygous recessive genotype aa is 36%. In activity a measuring blood pressure, students use a sphygmomanometer and stethoscope to measure blood pressure. When the necessary conditions are met, germination occurs and the rate of cellular respiration greatly increases. View notes ap bio lab 4 from bio 101 at university of arizona. Plot the results for the germinating seeds at 22c and 10c. At the end of 30 minutes remove the bags from the water. Molecular biology adapted from biorad forensic dna fingerprinting kit lab, vernier ap biology lab manual and college board ap biology lab manual. For the 201220 school year, the college board unveiled a new curriculum with a greater focus on scientific practices. Principles of natural selection and how natural selection drives evolution. Use a bactispreader to diffuse the cells over the entire surface of the plate, and dispose of the spreader. Teachers manual laboratory 8 3 time requirements required knowledge expectations objectives preparation laboratory 8. Ap bio lab 4 lab 4 plant pigments and photosynthesis. Founded in 1900, the college board was created to expand access to higher education.
How to write a lab proposal, formal lab, and primers on statistics. Enzyme catalysis alternative 2 this is another alternative to the ap enzyme catalysis lab. Select from an extensive catalog of educational tools available for enhancing the teaching of biological subjects, including simulated testing of blood and urine, the illustration of various natural habitats, and the dissection of sterilized stool samples collected from different regions. Paul andersen details the first 7 of labs in the ap biology curriculum. Bag contents initial mass final mass percent change in mass distilled water 0. He starts by discussing the process of transformation. Bigidea investigation 1 s17 evolution 1 investigation 1 artificial selection can extreme selection change expression of a quantitative trait in a population in one generation. Lab 8 genetics natalie cook ap biology population genetics.
At the end of 30 minutes remove the bag from the beaker, carefully blot it dry and determine its mass. Please give the answer and explain each step how to get it. Data collection for the class is best performed by asking for a show of hands. If you have ap credit, then you should enroll for a chm115 lab. The movement of a solute through a semi permeable memb rane is called dialysis as well as diffusion.
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